A NEUROTRANSMITTER'S (ACETYL CHOLINE) IN-VITRO OXIDATION AND ITS KINETIC STUDY BY USING CHLORAMINE- T(AN INFECTICIDAL AGENT) AS AN OXIDANT

Abstract

Acetylcholine and its analogs slow down the heart rate by producing the hyperpolarization in the pacemaker cells. Major effect of acetylcholine on heart function is to regulate its -function by decreasing the heart rate. The enzyme acetylcholinesterase diminishes its effect and breaks down acetylcholine into the inactive metabolites choline and acetate. Present project deals with the kinetic and mechanistic study of breakdown (oxidation) of acetylcholine chloride by chloramine-Tin HCI04 medium, which is spectrophotometrically monitored at Amax of 256 nm. The dependencies of the reaction rate on acetylcholine chloride and the reduction product of CAT, p-toluenesuifonamide, were studied. A mechanism consistent with the observed kinetic data has been proposed and discussed.